Last updated: 2020-10-28

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Knit directory: 20190717_Lardelli_RNASeq_Larvae/

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Rmd 6817b19 yangdongau 2020-10-28 build workflow
Rmd 417413a yangdongau 2020-10-28 Add in WGCNA and variance partitioning analysis
html e720795 yangdongau 2020-03-26 changes to visualize table
Rmd 443bb03 yangdongau 2020-03-26 Fix index
html 443bb03 yangdongau 2020-03-26 Fix index
Rmd 5099c07 yangdongau 2020-03-26 Index DE gene comparison and validating qPCR plot
html 5099c07 yangdongau 2020-03-26 Index DE gene comparison and validating qPCR plot
Rmd 6d48a07 yangdongau 2020-03-26 Index DE gene comparison analysis
html 7d1a0d3 yangdongau 2020-03-04 test
Rmd fa3b603 yangdongau 2020-03-04 1.Reorganize the files
html fa3b603 yangdongau 2020-03-04 1.Reorganize the files
html 7e50b3b yangdongau 2020-02-28 fix
html 8398869 yangdongau 2020-02-28 Merge branch ‘master’ of github.com:yangdongau/20190717_Lardelli_RNASeq_Larvae
html a19b0d3 yangdongau 2020-02-28 Change texts
html 3c8b6e1 yangdongau 2020-02-28 Add in annotations.
html 8f91594 yangdongau 2020-02-28 Add in annotations.
html 4e8adcc yangdongau 2020-02-28 change wrong work
html 91568d2 yangdongau 2020-02-28 Set KEGG diagram directory clean up the folder
html 0ce8f79 yangdongau 2020-02-27 clean up library packages
Rmd 3b63601 yangdongau 2020-02-27 index 3_GSEA.rmd
html 3b63601 yangdongau 2020-02-27 index 3_GSEA.rmd
Rmd 6c61705 yangdongau 2020-02-27 Compiled DE Gene analysis
html 6c61705 yangdongau 2020-02-27 Compiled DE Gene analysis
Rmd 84b88d5 yangdongau 2020-02-27 Initialised workflowr
html 84b88d5 yangdongau 2020-02-27 Initialised workflowr
Rmd 838c160 yangdongau 2020-02-27 Start workflowr project.

knitr::opts_chunk$set(
  autodep = FALSE,
  echo = TRUE,
  warning = FALSE,
  message = FALSE
)
library(tidyverse)

This website contains the analysis of RNAseq data from an experiment comparing 7 dpf zebrafish larvae from two genotypes. One genotype represented wild-type larvae, whilst the other genotype was a heterozygous mutation for the gene psen1, in which 6 nucleotides were deleted, to remove the two amino acids Q96 and K97. This mutation is generally referred to as psen1Q96K97.

Results from all steps can be found at:

  1. DE Gene Analysis
  2. Goseq Analysis & IRE enrichment test
  3. GSEA
  4. DE gene omparison with 6month brain and validating qPCR plot
  5. WGCNA
  6. Variance partition analysis

devtools::session_info()
─ Session info ──────────────────────────────────────────────────────────
 setting  value                       
 version  R version 3.6.0 (2019-04-26)
 os       macOS Mojave 10.14.6        
 system   x86_64, darwin15.6.0        
 ui       X11                         
 language (EN)                        
 collate  en_AU.UTF-8                 
 ctype    en_AU.UTF-8                 
 tz       Australia/Adelaide          
 date     2020-10-28                  

─ Packages ──────────────────────────────────────────────────────────────
 package     * version date       lib source        
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[1] /Library/Frameworks/R.framework/Versions/3.6/Resources/library